This group first appeared 20,000 years ago. It is the result of a genetic change in the Middle Eastern M96 populations who left Africa around 30,000 to 40,000 years ago. The Neolithic spread of these people out of the Middle East is associated with the marker M35 and now defines the Haplogroup E3b.
This group tends to appear in Italy, southeast Europe and North Africa. Migrating farmers where the likely cause of the spread, during the age of agricultural prosperity. This precipitated the geographical dispersal of M172 lineage.